猪低氧诱导因子1α(HIF1a)多克隆抗体
Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a)
[PRODUCT INFORMATION]
Immunogen: HIF1a, Porcine
Clonality: Clonality: Polyclonal
Host: Rabbit
Immunoglobulin mmunoglobulin Type: IgG
 Purification: Affinity Chromatography.
Applications  WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[IMMUNOGEN INFORMATION ]
Immunogen:  Recombinant HIF1a (Glu585~Asn824) expressed in E.coli. USCN 
Sequence: The target protein is fused with two N-terminal Tags, His-tag andGST-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERAEISMLEGAVLDIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALDVVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSDGS T S GS G H H H HH HSA G LVP R GS TAI GM KE T AAAK F E R Q HM DSP D LG TLEVLFQGPLGSEF- ESSSTS PQSTSTITVF QPTPMPEPPV TTTTPAATTD ELKTVAKDGMEDIKILIAFP SPPHVHKETT CATTSPYSDT GSRTASPNRA GKGVIEQTEK SHPRSPNVLSVALSQRTTVP EEELNPKIIA FQNAQRKRKI EHDGSLFQAV GIGTLLQQPD DRATTTSLSWKRVKGCKSSE QNGTEQKTII LIPSDLACRL LGQSMDESGL PQLTSYDCEV NAPIQGSRNLLQGEELLRAL DQVN
[ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against HIF1a. It has beenselected for its ability to recognize HIF1a in immunohistochemical staining andwestern blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user
[CONTENTS ] 
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.
[QUALITY CONTROL ] 
Content:The quality control contains recombinant HIF1a (Glu585~Asn824)disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%
[STORAGE ] 
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。