Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
产品名称： Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
英文名称： Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
产品编号： 10672ES03
产品价格： 0
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2024-12-10T13:14:19
使用范围： null
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Product Information
Product Name
Catalog No.
Size
Price (￥)
Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
10672ES03
1 U
105
10672ES10
10 U
645
10672ES60
100 U
4855
Product Description
This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.
This product is produced in accordance with GMP process requirements, and the product is provided in liquid form
Product Properties
Source
Recombinant E.coli with Pyrophosphatase gene from yeast
Optimum Temperature
25℃
Storage Buffer
50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% （v/v）TritonX-100, 50%（v/v）glycerin
Unit Definition
One unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.（The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min）
Contents
Catalog No.
Name
Catalog No./Specification
10672ES03
(1 U)
10672ES10
(10 U)
10672ES60
(100 U)
10672
Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
10 μL
100 μL
1 mL
Shipping and Storage
The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Notes
1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.
2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.
3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this
HB220701