PRKACA monoclonal antibody, clone 6D2.1
产品名称: PRKACA monoclonal antibody, clone 6D2.1
英文名称: PRKACA monoclonal antibody, clone 6D2.1
产品编号: MAB3591
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against synthetic peptide of PRKACA.
- Immunogen:
- A synthetic peptide corresponding to human PRKACA.
- Sequence:
- ESPAQNTAHLDQFERIK
- Host:
- Mouse
- Theoretical MW (kDa):
- 40
- Reactivity:
- Human
- Specificity:
- This antibody strongly reacts with human proteinkinase A catalytic (PRKACA) alpha subunit, and weakly with PKAc gamma subunit (both around 40 KDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
- Form:
- Liquid
- Concentration:
- 1 mg/mL
- Isotype:
- IgG
- Storage Buffer:
- In PBS, pH 7.4 (15 mM sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- New faces of the familiar clathrin lattice.
Wilbur JD, Hwang PK, Brodsky FM.Traffic. 2005 Apr;6(4):346-50.
- 2.
- Cargo- and compartment-selective endocytic scaffold proteins.
Szymkiewicz I, Shupliakov O, Dikic I.Biochem J. 2004 Oct 1;383(Pt 1):1-11.
- 3.
- Monoclonal antibody BF-06 against the heavy chain of clathrin.
Macurek L, Zikova M, Draberova E, Draber P.Folia Biol (Praha). 2003;49(6):238-40.
- Applications
- Dot Blot
- Dot Blot analysis of GST and GST-fusion proteins using PRKACA monoclonal antibody, clone 6D2.1 (Cat # MAB3591) and GST monoclonal antibody, clone S-tag-05 (Cat # MAB3651).
The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.
Lane 1 : GST-Akt1.
Lane 2 : GST-Akt2.
Lane 3 : GST-Akt3.
Lane 4 : GST-PKAc alpha.
Lane 5 : GST-PKAc beta.
Lane 6 : GST-PKAc gamma.
Lane 7 : GST-MEK 1.
Lane 8 : GST.
- Entrez GeneID:
- 5566
- Gene Name:
- PRKACA
- Gene Alias:
- MGC102831,MGC48865,PKACA
- Gene Description:
- protein kinase, cAMP-dependent, catalytic, alpha
- Omim ID:
- 601639
- Gene Ontology:
- Hyperlink
- Gene Summary:
- cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
- Other Designations:
- PKA C-alpha,cAMP-dependent protein kinase catalytic subunit alpha,cAMP-dependent protein kinase catalytic subunit alpha, isoform 1,protein kinase A catalytic subunit
- Gene Pathway
- Apoptosis
- Calcium signaling pathway
- Chemokine signaling pathway
- Gap junction
- GnRH signaling pathway
- Hedgehog signaling pathway
- Insulin signaling pathway
- Long-term potentiation
- MAPK signaling pathway
- Melanogenesis
- Olfactory transduction
- Prion diseases
- Taste transduction
- Vascular smooth muscle contraction
- Vibrio cholerae infection
- Wnt signaling pathway