FITC标记的泛素样蛋白NEDD8抗体
产品名称： FITC标记的泛素样蛋白NEDD8抗体
英文名称： Anti-NEDD8/FITC
产品编号： HZ-3812R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-NEDD8/FITC Conjugated antibody
FITC标记的泛素样蛋白NEDD8抗体
| 英文名称 | Anti-NEDD8/FITC |
| 中文名称 | FITC标记的泛素样蛋白NEDD8抗体 |
| 别 名 | NED8; NEDD 8; Neddylin; Neural precursor cell expressed developmentally down regulated 8; Neural precursor cell expressed developmentally down regulated gene 8; Rub1; Ubiquitin like protein Nedd 8; Ubiquitin like protein Nedd8. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 免疫学 神经生物学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 8.5kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NEDD8 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Ubiquitin like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12 and Hub1. Proteins of the second class include Parkin, RAD23 and DSK2, are designated ubiquitin domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog NEDD8 are activated by the E1 ubiquitin activating enzyme UBA2, that forms isopeptide linkages between thio esters. NEDD8 shows 80% homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3 ubiquitin ligase complex called the Skp1 /Cul1/Cdc53 F box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization. Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Subunit: Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53. Subcellular Location: Nucleus. Note=Mainly nuclear. Tissue Specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Post-translational modifications: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function. Similarity: Belongs to the ubiquitin family. Database links: Entrez Gene: 35151 Fruit fly (Drosophila melanogaster) Entrez Gene: 4738 Human Entrez Gene: 18002 Mouse Entrez Gene: 25490 Rat Entrez Gene: 368667 Zebrafish Omim: 603171 Human SwissProt: Q9VJ33 Fruit fly (Drosophila melanogaster) SwissProt: Q15843 Human SwissProt: P29595 Mouse SwissProt: Q71UE8 Rat Unigene: 30848 Fruit fly (Drosophila melanogaster) Unigene: 531064 Human Unigene: 296566 Mouse Unigene: 216415 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
泛素样蛋白分为两类：第一类，泛素样修饰物（UBLs）作为修饰剂，与泛素类似。UBLS的例子是SUMO、RUB1（也称为NEDD8）、APG12和HUB1。第二类蛋白质包括PARKIN、RAD23和DSK2，被称为泛素结构域蛋白（UDPs）。这些蛋白质含有与泛素有关的结构域，但彼此不相关。与UBLs相比，UDPs不被蛋白水解处理或与其他蛋白质结合。RUB1和相应的人类同源物NEDD8由E1泛素激活酶UBA2激活，从而形成硫酯之间的等肽链。NEDD8与泛素的同源性为80%。RuB1修饰的最有名的目标是cullin家族的成员。CulLIs是E3泛素连接酶复合物的亚基，称为Skp1/Cul1/CDC53 F盒（SCF）。SCF促进泛素从泛素缀合酶（E2）向靶蛋白的转移。RUB1修饰可调节SCF功能或定位。