上海中乔新舟生物科技有限公司是美国Sciencell公司官网公布的一级代理商：

       
       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。
 
请登录Sciencell公司官方网站（www.zqxzbio.com或www.sciencellonline.com）以确保购买正规公司产品。   

产品：小鼠肾系膜细胞

货号：M4200

Description

Renal mesangial cells are perivascular cells located within the central portion of the glomerular tuft between capillary loops, constituting 30-40% of the total glomerular cell population [1]. Mesangial cells synthesize, assemble and control turnover of the mesangial matrix [2]. They also regulate the intraglomerular capillary flow and ultrafiltration surface via mesangial cell contraction and release of various growth factors and vasoactive agents [3, 4]. By pinocytosis and phagocytosis, mesangial cells remove local accumulation of macromolecules in the mesangial space [5, 6]. Dysregulation of such housekeeping functions results in release of proinflammatory mediators and leukocyte infiltration, which subsequently affects glomerular permselectivity to cause proteinuria. Furthermore, mesangial cell proliferation and matrix overproduction have been observed in a wide variety of glomerular diseases, such as IgA nephropathy, mesangioproliferative glomerulonephritis, lupus nephritis, glomerulosclerosis and diabetic nephropathy [7, 8]. Such critical involvements suggest renal mesangial cells as an ideal model for studying mesangial injury and glomerular functions under both physiological and pathophysiological conditions.

MRMC from ScienCell Research Laboratories are isolated from neonatal day 2 rat renal tissue. MRMC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MRMC are characterized by immunofluorescence with antibodies specific to fibronectin, Thy-1, and smooth muscle actin. MRMC are negative for mycoplasma, bacteria, yeast and fungi. MRMC are guaranteed to further expand for 5 population doublings in the condition provided by ScienCell Research Laboratories.


Recommended Medium

It is recommended to use Mesangial Cell Medium (MCM, Cat. No. 4201) for the culturing of MRMC in vitro.


Product Use

MRMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.


Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.


Shipping

Dry ice.


Reference

[1] Olivetti G, Anversa P, Rigamonti W, et al. (1977) J Cell Biol. 75(2 Pt 1):573-85.
[2] Couchman JR, Beavan LA and McCarthy KJ. (1994) Kidney Int. 45:328-35.
[3] Riser BL, Cortes P, Heilig C, et al. (1996) Am J Pathol. 148:1915-23.
[4] Gruden G, Thomas S, Burt D, et al. (1999) J Am Soc Nephrol 10:730-37.
[5] Wanner C, Greiber S, Kramer-Guth A, et al. (1997) Kidney Int Suppl 63:S102-6.
[6] Gomez-Guerrero C, Suzuki Y and Egido J. (2002) Kidney Int 62:715-7.
[7] Schlöndorff D and Banas B. (2009) J Am Soc Nephrol. 20:1179-87.
[8] Abboud HE. (2012) Exp Cell Res. 318:979-85.