分子量:22.7 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human TFPI-2, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 2.5 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin, 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5.

产品特性:Human TFPI-2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 22.7 kDa. The predicted N-terminus is Asp 23. The reducing (R) protein migrates as 26-32 kDa in SDS-PAGE  due to glycosylation.

产品背景:Tissue Factor Pathway Inhibitor 2 (TFPI2) is also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF1), is a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). TFPI2 is highly abundant in the full-term placenta and widely expressed in umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. TFPI2 may play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa and has no effect on thrombin. Reduced synthesis of TFPI-2 has been related to numerous pathophysiological processes such as inflammation, angiogenesis, atherosclerosis, retinal degeneration and tumor growth / metastasis.