TRIM63 monoclonal antibody (M01), clone 6G6
产品名称: TRIM63 monoclonal antibody (M01), clone 6G6
英文名称: TRIM63 monoclonal antibody (M01), clone 6G6
产品编号: H00084676-M01
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant TRIM63.
- Immunogen:
- TRIM63 (NP_115977, 254 a.a. ~ 352 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- DKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGH
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
- MSDS:
- Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of TRIM63 expression in transfected 293T cell line by TRIM63 monoclonal antibody (M01), clone 6G6.
Lane 1: TRIM63 transfected lysate(40.2 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
- enlarge this image
- Immunofluorescence of monoclonal antibody to TRIM63 on HeLa cell . [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged TRIM63 is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of TRIM63 over-expressed 293 cell line, cotransfected with TRIM63 Validated Chimera RNAi ( Cat # H00084676-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM63 monoclonal antibody (M01), clone 6G6 (Cat # H00084676-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 84676
- GeneBank Accession#:
- NM_032588
- Protein Accession#:
- NP_115977
- Gene Name:
- TRIM63
- Gene Alias:
- FLJ32380,IRF,MURF1,MURF2,RNF28,SMRZ
- Gene Description:
- tripartite motif-containing 63
- Omim ID:
- 606131
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle. [provided by RefSeq
- Other Designations:
- OTTHUMP00000008701,iris ring finger protein,muscle specific ring finger protein 1,muscle specific ring finger protein 2,ring finger protein 28,striated muscle RING zinc finger protein